-
32 votes
-
Scientists use transcranial magnetic stimulation to make patients with chronic pain more hypnotizable
11 votes -
New material allows for better hydrogen-based batteries and fuel cells
17 votes -
Transparent wood is stronger than plastic and tougher than glass
28 votes -
Y'all are nerds (according to math)
8 votes -
Hacking the Climate - 37c3
7 votes -
Genetic engineering was meant to save chestnut trees. Then there was a mistake.
23 votes -
New study - scent of tears from female humans reduces revenge seeking and aggression in males, similar to patterns observed in other mammals
31 votes -
Making purple gold
26 votes -
Watch gravity pull two metal balls together
9 votes -
Reindeer combine sleeping and digesting, Norwegian researchers found after extracting reindeer brain data
9 votes -
What's the thinking out there on the fusion news that has been coming out?
8 votes -
World's first "self-amplifying" vaccine approved in Japan
15 votes -
The origin of mysterious green ‘ghosts’ in the sky has been discovered
18 votes -
Egyptian fractions and the greedy algorithm
6 votes -
Why scientists are making transparent wood
28 votes -
Wasabi linked to ‘substantial’ memory boost
28 votes -
Issues with NGS Library Prep
Greetings Folks, I apologize if this is the wrong spot for this but I'd like to cast a net to see if I can get any additional thoughts or help. I recently started a new job as NGS Library Prep...
Greetings Folks, I apologize if this is the wrong spot for this but I'd like to cast a net to see if I can get any additional thoughts or help.
I recently started a new job as NGS Library Prep Tech - sadly I had only begun training on this briefly at my last position but only got an introduction to Speed/Mag Bead clean up. I was hired because the lab is growing quickly and has had issues with organizational stuff in the past and that is my strong suit (my last position I did a lot of clonal DNA / miniprep stuff as far as the wet work went).
The person I was replacing at my new job was only there for two days and didn't really help a whole lot other than hand me a haphazardly written protocol and said "practice by cleaning ladders at different bead concentrations and running them on a gel."
Did that and was told they look good.
Fast forward to using actual samples: There were a set that needed to be redone because the final pool was lost. When I did my first qubit quants after the post PCR speed bead clean up I noticed that the quant concentrations were ~80% less than what they had been previously.
Today I have some remaining sample that I can push through PCR, my plan is to quant 8 out of the 53 samples of the pre / post PCR plate and then again after I do clean up.
As far as clean up goes I had been trying to do the whole plate at once, but I'm going to go back to just completing a column at a time to ensure my timing is better.
Are there particular spots in the Speed / Mag Bead clean up process that I should be aware that I could be washing away / losing DNA?
Do people have any tips on how to be more 'sure footed' in this process?
Ways that I can better practice and say "yup, I've got this!"?
Thanks for the help, and if this should be posted somewhere else please let me know, but as this is 'science' related I thought it fit best here.
7 votes -
Shocking study discovers bottlenose dolphins possess electric sixth sense
11 votes -
The business of bad medicine
4 votes -
Roar of cicadas was so loud, it was picked up by fiber-optic cables
11 votes -
Tiny robots made from human cells heal damaged tissue (in the lab)
15 votes -
What is a math department worth?
25 votes -
If you try to pass a bouncy ball under a table, if it hits the underside of the table it will just bounce back out the way it came
8 votes -
The achievement of gender parity in a large astrophysics research centre
7 votes -
The cocktail party effect — our stunning ability to filter out words and sounds
18 votes -
Vavilovian mimicry
10 votes -
What am I thankful for this year? Amazing scientific discoveries.
19 votes -
Machine learning creates a massive map of smelly molecules
14 votes -
Vanishing act for water waves - Perfect absorption cavity could protect coastlines
16 votes -
Mexican Congress holds second UFO session featuring Peruvian mummies
23 votes -
The Brain Scoop relaunch!
14 votes -
Something weird happens when you keep squeezing
19 votes -
Videoconference fatigue from a neurophysiological perspective (first neurophysiological evidence)
23 votes -
Deep in the Arctic permafrost, the Svalbard Global Seed Vault is protecting Africa's food supply
12 votes -
The story of when washing hands was considered crazy
12 votes -
Unzicker's "Real Physics": on dangers of Youtube physicists
12 votes -
In defense of the rat
14 votes -
Denmark is building on the success of blockbuster drugs – the country's focus on reinvestment is feeding a stream of discovery
7 votes -
Rats have an imagination, new research finds
57 votes -
The genetic heritage of the Denisovans may have left its mark on our mental health
16 votes -
Long presumed to have no heads at all, sea stars may be nothing but
25 votes -
A brief history of tricky mathematical tiling
10 votes -
What causes fainting? Scientists finally have an answer.
22 votes -
Scientists in Sweden have succeeded in extracting and sequencing RNA molecules from an extinct species, a century old Tasmanian tiger known as a thylacine
16 votes -
Six creatures that are actually real-life zombies
18 votes -
'Not of faculty quality': How Penn mistreated Katalin Karikó, the Nobel Prize winner of 2023
25 votes -
Maths anxiety
12 votes -
How laboratory glassware is blown in the UK
12 votes -
Future technology: Twenty-two ideas about to change our world
6 votes