Greetings Folks, I apologize if this is the wrong spot for this but I'd like to cast a net to see if I can get any additional thoughts or help.

I recently started a new job as NGS Library Prep Tech - sadly I had only begun training on this briefly at my last position but only got an introduction to Speed/Mag Bead clean up. I was hired because the lab is growing quickly and has had issues with organizational stuff in the past and that is my strong suit (my last position I did a lot of clonal DNA / miniprep stuff as far as the wet work went).

The person I was replacing at my new job was only there for two days and didn't really help a whole lot other than hand me a haphazardly written protocol and said "practice by cleaning ladders at different bead concentrations and running them on a gel."

Did that and was told they look good.

Fast forward to using actual samples: There were a set that needed to be redone because the final pool was lost. When I did my first qubit quants after the post PCR speed bead clean up I noticed that the quant concentrations were ~80% less than what they had been previously.

Today I have some remaining sample that I can push through PCR, my plan is to quant 8 out of the 53 samples of the pre / post PCR plate and then again after I do clean up.

As far as clean up goes I had been trying to do the whole plate at once, but I'm going to go back to just completing a column at a time to ensure my timing is better.

Are there particular spots in the Speed / Mag Bead clean up process that I should be aware that I could be washing away / losing DNA?

Do people have any tips on how to be more 'sure footed' in this process?

Ways that I can better practice and say "yup, I've got this!"?

Thanks for the help, and if this should be posted somewhere else please let me know, but as this is 'science' related I thought it fit best here.

Hey there! Back when the pandemic was in full swing, I stumbled upon a comment that shared a link to a website with a title quite like this post. I can't quite recall if I saw the comment on Reddit, the orange site, or even here. The site was quite basic, and claimed to have a list of jobs from companies that understood that its workers would like to have a life outside of work

The job market has changed a lot since the pandemic, but if any of you awesome folks happen to know where I can find a good part-time software development job, I'd be seriously grateful.

We all have things we hate about our jobs — for many of us it’s the actual job itself — but I’m interested in hearing about the things that you actually like.

They can be big, satisfying things (like the fulfillment of completing a big project) or they can be tiny little insignificant joys (like writing your to-do list with a really smooth pen).

Let us know what you genuinely like about your work.

I am helping someone look for a job related to coding (in Canada). Software development, web development, app development, etc. You get the idea. I have no connections in tech to help them network. And I don't understand what the difference is between an intermediate/intro position, what qualifications to help them highlight on their resume, or what is preventing them from finding a job. I know they've applied to hundreds of posts on LinkedIn and Indeed, etc. but they have only received a single interview (but no job).

I am at a loss - how do I help this person? How do you help someone find a job in a field you don't understand or have any network to help them meet the right person? Tech specific advice would be a godsend, but really any job-search related tricks, tips, etc. that I can pass on would be greatly appreciated.

First of all, this is a lot about me and myself and I'm sorry it's a bit self-centered; it's been bouncing around my head and I want to get it out somewhere. Please let me know if this isn't appropriate here.

Secondly, teachers or those in training to become one: I want to hear your thoughts on this question.

Why am I becoming a teacher?

I've been finding that I'm asking this question of myself a lot lately. My goal is and always has been the same for years: I want to teach, I feel good teaching, I feel I have a purpose and that purpose has been what's driven me forward when I wanted to give up. Truly though - why do I want to be a teacher?

I could do the same style of work in other settings. I could become a tutor, self-employed or otherwise, and assist students in a specific capacity. I could be a YouTuber, creating video essays on self-researched subjects of passion. I could be a writer, bringing the same content through literature to a wholly different audience. In all of these, there is the potential to make more money, reach a wider audience, and leave a more indelible impact upon the world.

So, why am I becoming a teacher?

15 years ago, I dropped out of college, suffering depression. I wasn't the only one depressed; aside from the millions of others reeling from mental health issues, the economy was entering a recession in 2008. I was a NEET - jobless, out of school, and seemingly stuck. My family (read: my dad, stepmom, and sisters) had abandoned me - they had other matters to worry about than their wayward son - and I was fortunate my mother whom I'd dissociated from years before reached out to me. With her help, I got back on my feet, moved across the country, and began looking for work with slight hope. I volunteered one day to read at the school she worked at, and the teacher in the room went to the admins and demanded I be hired on the spot. I was.

Thus began a journey of discovery. I was good at something, and I felt good about doing it. I felt something to replace my depression and self doubt: worthiness.

Over the years, I honed my craft and continued sporadically attending school - when I could afford it - in order to become able to lead my own classroom in our private school/daycare. That was 7 years ago, and I've been teaching prek (4-5 year olds) since then. I'm able to teach reading, writing, mathematics, chess, life lessons, history, biology, astronomy, geology, entomology... the list goes on and on. I have a passion for learning, and for sharing that learning.

Is that why I am becoming a teacher?

The biggest obstacle to achieving my ultimate dream - teaching in public schools - was always the degree. I had dropped out of college twice - in 2008 and again in 2013 - before finally completing an Associates degree in 2016. I felt that, financially, getting my bachelor's would never happen. Massive student loan debt (private debt north of $30k) and low wages in childcare meant I wasn't getting anywhere. Life changes though, and the stars aligned - the private debt was written off, I got out of defaulting on my federal loans, and just in time to qualify for a state program to get me in school again and have a full ride scholarship. It was happening!

Now we live in a post-pandemic world... Do I still want to become a teacher?

At first, attitudes were siding with teachers. There was sympathy for their struggles and worries, the low pay and high barrier to entry. That quickly changed, as it did for medical workers and others in the pandemic world. Teachers struggle more now than they have before. Fewer resources, more troubled students that desperately need help, more resistance from parents and communities trying to prove that teachers and schools aren't necessary in the way they have been, and more burnout and shortages across the nation.

I see all this and yet I press on. Why?

The thing is, I'm not sure. My resolve is strong and I've been persistent and diligent in my schooling. I've worked too long and hard to give up this opportunity. Why do I still want to teach, though? Why not find an administrative job with potentially more pay and better work environment? Why not leave education altogether and use my skills elsewhere?

It comes back to what drove me forward in the first place: purpose. I feel in direct connection with the future by doing what I do. I feel like in some miniscule, imperceptible, but meaningful way, I can help create a better world tomorrow by doing what I do today. It gives my life meaning, and nobody and nothing can take that from me. I've changed hundreds, potentially thousands, of lives already. Students return years later to tell how much I meant to them - these are students I had known at ages 4 and 5 who still remember me a decade later!

So, why am I becoming a teacher?

Because someone has to do it, and that someone might as well be me. I enjoy my work, I enjoy the ups and downs, I enjoy the struggles and challenges and overcoming them, I enjoy making difficult topics understandable to young minds, I enjoy what I do even when I hate it. To me, that's love.

With good luck and a positive outlook, I'll be graduating with a degree in Early Childhood Education next September. It may not be prestigious, it may not make me a lot of money, but it will allow me to continue on the path I've set myself. Thanks for reading.

I've been a professional in the IT sector for the past 25 years, and during that time I've gone through several different methods of finding my next gig. Back when I started out, the internet was still a relatively new thing, so I got my first few positions by answering ads in the local newspaper (remember those?)

Two years ago, I decided to try my hand at writing novels, and while that has been quite fulfilling personally, it hasn't yet started to pay any bills so I've had to keep my IT skills sharp and hold down a standard job to pay the bills.

Now though, I find that I'm looking a lot harder at the companies and people I work for, and I'd like to be able to shop around a bit more for a position at a place that is more in line with me as a person.

To that end, I'm wondering what methods are more commonplace now for finding employment, as opposed to my standard, which is pretty much indeed and the occasional linkedin find. Which methods have you had the most success with?

https://www.sciencedirect.com/science/article/pii/S1048984322000583

Abstract

We submitted over 12,000 job applications, to over 4,000 job advertisements, to investigate hiring discrimination against six ethnic groups for leadership positions.

For leadership positions, applicants with English names received 26.8% of positive responses for their job applications, while applicants with non-English names received 11.3% of positive responses. This means ethnic minorities received 57.4% fewer positive responses than applicants with English names for leadership positions despite identical resumes.

For non-leadership positions, applicants with English names received 21.2% of positive responses for their job applications, while applicants with non-English names received 11.6% of positive responses. This means ethnic minorities received 45.3% fewer positive responses for non-leadership positions despite identical resumes.

Ethnic discrimination for leadership positions was even more pronounced when the advertised job required customer contact.

For example, this article shows demand for aviation mechanics. What are your observations and experiences re demand in hiring?

https://apnews.com/article/jobs-workers-shortages-hiring-manufacturing-airplane-mechanics-84176a760eaacd1b629e9cef2f6ee42c

Question is for our users here who work/worked in the tech industry (in any capacity) or in a techy position in any industry.

What have you learned?
How did it change you?

Previous questions in series:

What have you learned from...
...being a parent?
...going through a breakup?
...moving to a new place?

These threads remain open, so feel free to comment on old ones if you have something to add!

First off, fuck job applications. It's an awful and tedious charade. Creating accounts on hundreds of websites for the resume parser to not work and have to manually upload that all again, to then write a cover letter that's skimmed at best, for a word to be missing from the resume which their detection tech passes before you're given a real shot.

But regardless that's not why I'm here. I'm in the process of applying to jobs, but for the first time I'm applying to jobs internationally (I'm US based). Have any of y'all applied for and received jobs abroad? What was successful and what wasn't? I'm primarily looking into pharmaceutical research or pharmacovigilance/drug safety because that's where English language jobs are in my area of study, but hope to eventually become fluent enough in a different language so I can move back into infection prevention or disease surveillance.